Rat cytochrome P450-mediated transformation of dichlorodibenzo-p-dioxins by recombinant white-rot basidiomycete Coriolus hirsutus
نویسندگان
چکیده
منابع مشابه
Transformation of the white-rot basidiomycete Coriolus hirsutus using the ornithine carbamoyltransferase gene.
An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg+ with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 10(3)-1...
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Industrially produced carbon-based nanomaterials (CNM), including fullerenes and nanotubes, will be introduced into the environment in increasing amounts in the next decades. One likely environmental chemical transformation of C60 is oxidation to C60 fullerol through both abiotic- and biotic-mediated means. Unfortunately, knowledge of the environmental fate of oxidized CNM is lacking. This stud...
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K A T H R Y N M . S C H R E I N E R , † T I M O T H Y R . F I L L E Y , * , † R O B E R T A . B L A N C H E T T E , ‡ B R E N D A B E I T L E R B O W E N , † R O B E R T D . B O L S K A R , § W I L L I A M C . H O C K A D A Y , | C A R O L I N E A . M A S I E L L O , | A N D J A M E S W . R A E B I G E R § Department of Earth and Atmospheric Sciences, Purdue University, West Lafayette, Indiana ...
متن کاملInvolvement of Cytochrome P450 in Pentachlorophenol Transformation in a White Rot Fungus Phanerochaete chrysosporium
The occurrence of cytochrome P450 and P450-mediated pentachlorophenol oxidation in a white rot fungus Phanerochaete chrysosporium was demonstrated in this study. The carbon monoxide difference spectra indicated induction of P450 (103±13 pmol P450 per mg protein in the microsomal fraction) by pentachlorophenol. The pentachlorophenol oxidation by the microsomal P450 was NADPH-dependent at a rate ...
متن کاملDegradation of 2,7-dichlorodibenzo-p-dioxin by the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Under secondary metabolic conditions, the white-rot basidiomycete Phanerochaete chrysosporium degraded 2,7-dichlorodibenzo-p-dioxin (I). The pathway for the degradation of I was elucidated by the characterization of fungal metabolites and oxidation products generated by lignin peroxidase (LiP), manganese peroxidase (MnP), and crude intracellular cell-free extracts. The multistep pathway involve...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2005
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-005-1943-0